Title: THERMOPLASTIC MICROFLUIDIC PCR TECHNOLOGIES FOR NEAR PATIENT DIAGNOSTICS
Date: Thursday, June 15
Time: 2:00 PM
Location: DeWALT seminar room (Glenn L. Martin Hall)
Dr. Ian White – Dean’s Representative
Finally, a 768-element staggered trap array is demonstrated, with highly reliable passive loading and discretization achieved within 5 min. Finally, a technique for reagent integration by pin spotting affords simplified workflow, and the ability to perform multiplexed PCR. Reagent printing formulations were optimized for stability and volume consistency during spotting. Paraffin wax was demonstrated as a protective layer to prevent rehydration and reagent cross contamination during sample loading. Deposition was accomplished by a custom pin spotting tool. A staggered trap array device with integrated reagents successfully amplified and validated a 2-plex assay, showing the potential of the platform for a multiplexed antibiotic resistance screening panel.