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Alex Sposito Defense June 15, 2017

Title: THERMOPLASTIC MICROFLUIDIC PCR TECHNOLOGIES FOR NEAR PATIENT DIAGNOSTICS

Date: Thursday, June 15

Time: 2:00 PM

Location: DeWALT seminar room (Glenn L. Martin Hall)

Committee:
Dr. Don DeVoe – Chair
Dr. Miao Yu
Dr Benjamin Shapiro
Dr. Sarah Bergbreiter

Dr. Ian White – Dean’s Representative

Abstract:Microfluidic technologies have great potential to help create portable, scalable, and cost-effective devices for rapid polymerase chain reaction (PCR) diagnostics in near patient settings. Unfortunately, current PCR diagnostics have not reached ubiquitous use in such settings because of instrumentation requirements, operational complexity, and high cost. This dissertation demonstrates a novel platform that can provide reduced assay time, simple workflow, scalability, and integration in order to better meet these challenges. First, a disposable microfluidic chip with integrated Au thin film heating and sensing elements is described herein. The system employs capillary pumping for automated loading of sample into the reaction chamber, combined with an integrated hydrophilic valve for precise self-metering of sample volumes into the device. With extensive multiphysics modeling and empirical testing we were able to optimize the system and achieve cycle times of 14 seconds and completed 35 PCR cycles plus HRMA in a total of 15 minutes, for successful identification of a mutation in the G6PC gene indicative of von Gierke’s disease.

Next, a scalable sample digitization method that exploits the controlled pinning of fluid at geometric discontinuities within an array of staggered microfluidic traps is described. A simple geometric model is developed to predict the impact of device geometry on sample filling and discretization, and validated experimentally using fabricated cyclic olefin polymer devices.

Finally, a 768-element staggered trap array is demonstrated, with highly reliable passive loading and discretization achieved within 5 min. Finally, a technique for reagent integration by pin spotting affords simplified workflow, and the ability to perform multiplexed PCR. Reagent printing formulations were optimized for stability and volume consistency during spotting. Paraffin wax was demonstrated as a protective layer to prevent rehydration and reagent cross contamination during sample loading. Deposition was accomplished by a custom pin spotting tool. A staggered trap array device with integrated reagents successfully amplified and validated a 2-plex assay, showing the potential of the platform for a multiplexed antibiotic resistance screening panel.

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link

 

[gview file=”https://wordpress.umd.edu/grad/wp-content/uploads/sites/4/2017/05/Ad-for-UCB-and-HERC.pdf”]

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M.S. Thesis Defense – R. Sridharan

Title: Stress Response of Tall and Heavy Electronic Components Subjected to Multi-axial Vibration

Day: February 24, 2017

Time: 10:30am

Location: EGR 2164

Committee:

Professor Abhijit Dasgupta

Professor Miao Yu

Professor Amr Baz

Abstract:

Electronic assemblies often experience multiaxial vibration environments in use and tall, heavy components are more vulnerable to such multiaxial vibration than are shorter, lighter assemblies.  The added vulnerability comes from higher stresses that are a result of nonlinear dynamic amplification which large components are susceptible to under simultaneous multiaxial excitation, termed multi degree of freedom (MDoF) excitation.  However, it is still common practice to conduct vibration durability testing on electronic assemblies one axis  at a time – in what is termed sequential single degree of freedom (SSDoF) testing.  SSDoF testing has been shown to produce lower fatigue damage accumulation rates than simultaneous MDoF testing, in the leads of tall and heavy electronic components.  This leads to overestimating the expected lifespan of the assembly.

This paper investigates the geometric nonlinearities and the resulting cross-axis interactions that tall and heavy electronic components experience when subjected to vibration excitation along two orthogonal axes – one direction is in the plane of the PWB and the other is along the normal to the PWB.  The direction normal to the PWB aligns with the axial direction of the leads, while the in-plane direction aligns with the primary bending direction of the leads.  Harmonic excitation was simultaneously applied to both axes to study the vibration response as a function of: frequency ratio, phase difference and amplitude of the excitation along the two axes.    The experimental observations were verified with a nonlinear dynamic Finite Element study.  The effect of geometric nonlinearity on cyclic stresses seen in the vibrating component are analyzed.

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National Science Foundation Scholarships in Science, Technology, Engineering, and Mathematics – 2016

There are few scholarships available with National Science Foundation. Please find attached document for more details.

[gview file=”https://wordpress.umd.edu/grad/wp-content/uploads/sites/4/2016/12/nsf17527.pdf”]

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Faculty Positions in Mechanical/Aerospace Engineering at The University of Texas at El Paso

Dear Colleagues,

The Mechanical Engineering Department at the University of Texas at El Paso (UTEP) announces tenure-track and tenured faculty positions in areas encompassed by mechanical and aerospace engineering. Opportunities exist at the Assistant and Associate Professor ranks. The Department’s long term plan for these positions focuses on composite materials, aerospace structures, and additive manufacturing but other concentration areas are acceptable. Strong applicants are expected to describe how their fields augment the departmental goals.

The Department of Mechanical Engineering at UTEP has an enrollment of over 1100 graduate and undergraduate students. The Department has a dynamic research programs, with a more than 500% increase in annual research expenditures over the last 5 years. The Department houses two of UTEP’s top research centers, the NASA MIRO Center for Space Exploration and Technology Research and the W. M. Keck Center for 3D Innovation. The Department’s research and education programs strive to create abundant opportunities for students and to bring the Southwest border region to the pinnacle of advanced technology research excellence. The current research portfolio of the Mechanical Engineering Department includes a broad range of federally and industry-funded projects that support research activities in three major areas: aerospace and defense engineering, additive manufacturing, and energy systems. The Department has world class research facilities in the areas of advanced manufacturing, aerospace systems, and energy engineering. The Department is currently building a large technology research and innovation acceleration facility at an external site in Fabens, Texas. The Department also has exceptional computational capabilities as well as state-of-the-art manufacturing and design practice facilities. 

The Department offers an ABET-accredited B.S. degree in Mechanical Engineering, an M.S. degree in Mechanical Engineering, and a Ph.D. in Mechanical Engineering. The Department is currently developing a proposal to offer an M.S. degree in Aerospace Engineering.  The Department also participates in interdisciplinary Ph.D. programs in Materials Science and Engineering, Environmental Science and Engineering, and Computational Science.  The Department is part of a rapidly growing College of Engineering that has doubled its research expenditures and experienced a 25% increase in enrollment over the last five years. Additional information about the Department and College is available at http://me.utep.edu and http://engineering.utep.edu.

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ASHRAE Field Trip Announcement: EVAPCO

Dear All:

The ASHRAE UMD Student Branch will visit the Corporate Headquarters and Research Center of EVAPCO, Inc. on Friday, November 11. Transportation and lunch will be provided. This trip is open to all UMD students.
The site visit will provide an overview of EVAPCO and a tour of the 60,000 square foot research facilities including environmental and 3D printing labs. Space is limited – please RSVP by Friday, November 4, 12pm noon, and reserve 10:15am-5:00pm on November 11 for the site visit. Please find attached flyer for more details.
[gview file=”https://wordpress.umd.edu/grad/wp-content/uploads/sites/4/2016/11/ASHRAE-Field-Trip-EVAPCO-Nov-11.pdf”]
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RAP Review

There are outstanding fellowship opportunities in the National Academies of Sciences, Engineering, and Medicine.  More detailed information and an online application can be found at www.nationalacademies.org/rap.

The National Research Council of the National Academies sponsors a number of awards for graduate, postdoctoral and senior researchers at participating federal laboratories and affiliated institutions. These awards include generous stipends ranging from $45,000 – $80,000 per year for recent Ph.D. recipients, and higher for additional experience.  Graduate entry level stipends begin at $30,000.  These awards provide the opportunity for recipients to do independent research in some of the best-equipped and staffed laboratories in the U.S.  Research opportunities are open to U.S. citizens, permanent residents, and for some of the laboratories, foreign nationals.

Detailed program information, including online applications, instructions on how to apply, and a list of participating laboratories, are available on the NRC Research Associateship Programs Web site (see link above).

 Questions should be directed to the NRC at 202-334-2760 (phone) or rap@nas.edu.

There are four annual review cycles.

Review Cycle:  November; Opens September 1; Closes November 1

Review Cycle:  February; Opens December 1; Closes February 1

Review Cycle:  May; Opens March 1; Closes May 1

Review Cycle:  August; Opens June 1; Closes August 1

 

Applicants should contact prospective Adviser(s) at the lab(s) prior to the application deadline to discuss their research interests and funding opportunities.

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Frank VanGessel Masters Thesis Defense. April 12, 2016.

Title: Fully Anisotropic Solution of the Three Dimensional Boltzmann Transport Equation

Date: April 12, 2016

Time: 1:00 p.m.

Location: DeWalt Seminar Room EGR2164

Committee Members:

Dr. Peter Chung

Dr. Abhijit Dasgupta

Dr. Bao Yang

Abstract: 

The development of accurate modeling techniques for nanoscale thermal transport is an active area of research. Modern day nanoscale devices have length scales of tens of nanometers and are prone to overheating, which reduces device performance and lifetime. Therefore, accurate temperature profiles are needed to predict the reliability of nanoscale devices. The majority of models that appear in the literature obtain temperature profiles through the solution of the Boltzmann transport equation (BTE). These models often make simplifying assumptions about the nature of the quantized energy carriers (phonons). Additionally, most previous work has focused on simulation of planar two dimensional structures. This thesis presents a method which captures the full anisotropy of the Brillouin zone within a three dimensional solution to the BTE. The anisotropy of the Brillouin zone is captured by solving the BTE for all vibrational modes allowed by the periodic boundary conditions.

 

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Graduate Intern- structural dynamics

Please find the details here:

https://www.usajobs.gov/GetJob/ViewDetails/433692400